A 2.00-mL solution of apotransferrin was titrated as illustrated in Figure 17-10. It required 163 L of

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A 2.00-mL solution of apotransferrin was titrated as illustrated in Figure 17-10. It required 163 L of 1.43 mM ferric nitrilotriacetateto reach the end point.

(a) Why does the slope of the absorbance-versus-volume graph change abruptly at the equivalence point?

(b) How many moles of Fe(III) (= ferric nitrilotriacetate) were required to reach the end point?

(c) Each apotransferrin molecule binds two ferric ions. Find the concentration of apotransferrin in the 2.00-mL solution.

Figure 17-10

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Quantitative Chemical Analysis

ISBN: 978-1429218153

8th edition

Authors: Daniel C. Harris

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Question Posted: Apr 19, 2016 10:02 AM

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A 2.00-mL solution of apotransferrin was titrated as illustrated in Figure 17-10. It required 163 L of

Question:

End point 0.400 0.300 0.200 0.100 100 200 300 ul of Fe(lll) added Corrected absorbance at 465 nm

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a Prior to the equivalence point all added Fe III binds to the protei...View the full answer

Quantitative Chemical Analysis

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