A layer with negligible thickness containing 10.0 nmol of methanol (D = 1.6 10 - 9

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A layer with negligible thickness containing 10.0 nmol of methanol (D = 1.6 × 10-9 m2/s) was placed in a tube of water 5.00 cm in diameter and allowed to spread by diffusion. Using Equation 22-25, prepare a graph showing the Gaussian concentration profile of the methanol zone after 1.00, 10.0, and 100 min. Prepare a second graph showing the same experiment with the enzyme ribonuclease (D = 0.12 × 10-9 m2/s).


Equation 22-25

Broadening of chromatography band by diffusion: m (22-25) c = V4TDT

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