Although a large number of mutagenic chemicals are known, none is known that induces mutations in only a single gene (gene-specific mutagenesis). From what you know about mutagens, explain why it is unlikely that a gene-specific chemical mutagen will be found. How then is site-specific mutagenesis accomplished?
Answer to relevant QuestionsWhy is it difficult in a single experiment to transfer a large number of genes to a recipient cell using transformation or transduction? Describe the similarities and differences between expression vectors and shuttle vectors. How has metagenomics been used to find novel useful products? How are gene fusions used to investigate gene regulation? What is fitness? To what degree does fitness depend on the environment in which organisms live?
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