Amino acid compositions can be determined by heating a protein in 6 M HCl and running the hydrolysate through an ion-exchange column. If you were going to do an amino acid sequencing experiment, why would you want to get an amino acid composition first?
Answer to relevant QuestionsHow could you isolate mitochondria from liver cells using differential centrifugation? What are the main procedures involved in a western blot? What are some of the assumptions behind the logic of the experiment described in figure 5.26? How can gel-filtration chromatography be used to arrive at an estimate of the molecular weight of a protein? Distinguish between the lock-and-key and induced-fit models for binding of a substrate to an enzyme.
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