An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cation-exchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?
Answer to relevant QuestionsWhat is meant by “salting out”? How does it work? For an enzyme that displays Michaelis–Menten kinetics, what is the reaction velocity, V (as a percentage of Vmax), observed at the following values? (a) [S] 5 KM (b) [S] 5 0.5KM (c) [S] 5 0.1KM (d) [S] 5 2KM (e) [S] 5 10KM For the reaction of glucose with oxygen to produce carbon dioxide and water, Glucose + 6O2 → S 6CO2 + 6H2O The DG° is 22880 kJ mol –1, a strongly exergonic reaction. However, a sample of glucose can be maintained ...Draw Lineweaver–Burk plots for the behavior of an enzyme for which the following experimental data are available. Explain how phosphorylation is involved in the function of the sodium–potassium ATPase.
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