An article in the Electronic Journal of Biotechnology (Optimization of Medium Composition for Transglutaminase Production by a

An article in the Electronic Journal of Biotechnology (€œOptimization of Medium Composition for Transglutaminase Production by a Brazilian Soil Streptomyces sp.,€) describes the use of designed experiments to improve the medium for cells used in a new microbial source of transglutaminase (MTGase), an enzyme that catalyzes an acyl transfer reaction using peptide-bond glutamine residues as acyl donors and some primary amines as acceptors. Reactions catalyzed by MTGase can be used in food processing. The article describes two phases of experimentation: screening with a fractional factorial, and optimization. We will use only the optimization experiment. The design was a central composite design in four factors: x1 = KH2PO4, x2 = MgSO47H2O, x3 = soybean flour, and x4 = peptone. MTGase activity is the response, which should be maximized. Table 14E.11 contains the design and the response data.

The design and data are in the Minitab worksheet Ex14-18.MTW.

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TABLE 14E. 11 The MTGase Optimization Experiment for Exercise 14.18 MTGase x4 Activity Standard Order 0.87 0.74 051 0.99 0.67 0.72 0.81 101 1.33 0.7 0.82 0.78 0.36 0.23 0.21 0.44 0.56 0.49 0.57 0.81 0.9 0.65 0.91 0.49 143 1.17 1.5 I1 3 51 7 9-1 12 13-1 14 15 1 1 172 18 19 20 21 0 0 0 0-2 0 0 02 0 24 0 26 27