Hydrazine cleaves amide

Hydrazine cleaves amide bonds to form acylhydrazides according to the general mechanism of nucleophilic acyl substitution discussed in Chapter 20:
This reaction forms the basis of one method of terminal residue analysis. A peptide is treated with excess hydrazine in order to cleave all the peptide linkages. One of the terminal amino acids is cleaved as the free amino acid and identified; all the other amino acid residues are converted to acylhydrazides. Which amino acid is identified by hydrazinolysis, the N terminus or the C terminus?

Sales 0
Views 1034
Comments 0
  • CreatedDecember 29, 2009
  • Files Included