Enzyme-catalyzed reactions are sometimes analyzed by use of the Eadie-Hofstee plot, in which v is plotted against v/[S]o
(a) Using the simple Michaelis-Menten mechanism, derive a relation between v/[S]o and v.
(b) Discuss how the values of K M and v max, are obtained from analysis of the Eadie-Hofstee plot.
(c) Determine the Michaelis constant and the maximum velocity of the reaction of the reaction from Problem 23.23 by using an Eadie-Hofstee plot to analyze the data.