Explain why a certain protein has an apparent molecular mass of 90 kD when determined by gel

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Explain why a certain protein has an apparent molecular mass of 90 kD when determined by gel filtration and 60 kD when determined by SDS-PAGE in the presence or absence of 2-mercaptoethanol.Which molecular mass determination is more accurate?
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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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