Explain why an Escherichia coli strain that is His− is an auxotroph and one that is Lac− is not.
Answer to relevant QuestionsWhat are silent mutations? From your knowledge of the genetic code, why do you think most silent mutations affect the third position in a codon? What is a sex pilus and which cell type, F− or F+, would produce this structure? Describe two prokaryotic cloning hosts and the beneficial and detrimental features of each. How has metagenomics been used to find novel useful products? Suppose you are given the task of constructing a plasmid expression vector suitable for molecular cloning in an organism of industrial interest. List the characteristics such a plasmid should have. List the steps you would ...
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