From chapter 9, figure 9.24 d. Use the technology from figure 10.11 as your guide to arrive at the same result as shown here, but without the mouse.

Figure 10.11

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Live R strain Heat-killed S strain Dies Live S and R strains isolated from dead mouse Isolated gene 70000 1 The selected gene (blue) is isolated 2 Selection of cloning vector from the chromosome of a genetic donor. red plasmid) with correct restriction sites. Donor gene and vector are exposed to the same restriction endonucease EcoRi which cuts at sites GAATTO CTTAAG nn Section of Vector donor gene to be cloned ends bind to form a single recombinant plasmid. 5 Transformation. The recombinant plasmid is introduced into cloning host bacteria. INSIDE THE CELL IN THE LAB cloning host DNA The transformed cell is increase numbers. Every cell contain a replicated plasmid containing the desired gene grown in culture to Multiply in medum Donor Ce s Cels are induced to transcribe and translate the donor gene. MRNA This yields a protein product (such as alpha-2a interferon) that is recovered from the culture Protein product