Give an example of a scenario in which you could partially isolate a protein with differential centrifugation using only one spin.
Answer to relevant QuestionsYou are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate pre-cipitation as the next step. Knowing ...Why do most people elute bound proteins from an ion-exchange column by raising the salt concentration instead of changing the pH? An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cation-exchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino ...The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase: CO2 + H2O → HCO-3 + H+ [H. De Voe and G. B. ...Why can we say that having a pure non- competitive inhibitor present is similar to just having less enzyme present?
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