Give an example of one biological, one chemical, and one physical mutagen and describe the mechanism by which each causes a mutation.
Answer to relevant QuestionsWhat are heteroduplex regions of DNA and what process leads to their formation? Although a large number of mutagenic chemicals are known, none is known that induces mutations in only a single gene (gene-specific mutagenesis). From what you know about mutagens, explain why it is unlikely that a ...How has bacteriophage T7 been used in expressing foreign genes in Escherichia coli, and what desirable features does this regulatory system possess? Describe the basic principles of gene amplification using the polymerase chain reaction (PCR). How have thermophilic and hyperthermophilic prokaryotes simplified the use of PCR? Many genetic systems use the lacZ gene encoding β-galactosidase as a reporter. What advantages or problems would there be if (a) luciferase or (b) green fluorescent protein were used instead of β-galactosidase as ...
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