How can a phylogenetic picture of a microbial community be obtained without culturing its inhabitants?
Answer to relevant QuestionsAfter PCR amplification of total community DNA using a specific primer set, why is it necessary to either clone or run DGGE on the products before sequencing them? How and in what way does an input of organic matter, such as sewage, affect the oxygen content of a river or stream? The surface of a rock in a flowing stream will often contain a biofilm. What advantages could be conferred on bacteria growing in a biofilm compared with growth within the flowing stream? Which group of bacteria cycle sulfur compounds under anoxic conditions? If sulfur chemolithotrophs had never evolved, would there be a problem in the microbial cycling of sulfur compounds? Which organic sulfur compounds are ...Which Bacteria and Archaea play a major role in acid mine drainage? Why do they carry out the reactions that they do? Why is air necessary for this process?
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