How could you isolate mitochondria from liver cells using differential centrifugation?
Answer to relevant QuestionsWhich enzymes or chemicals would you choose to use to cut the protein from Question 50? Why? Where did western blot get its name? Give an example of a scenario in which you could partially isolate a protein with differential centrifugation using only one spin. What is the main difference between reverse phase HPLC and standard ion-exchange or gel filtration chromatography? Using an energy diagram, show why the lock-and-key model could lead to an inefficient enzyme mechanism.
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