How does HPLC differ from ion-exchange chromatography?
Answer to relevant QuestionsDesign an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0. Would you use a pH meter to monitor the progress of the reaction described in Question 14? Why or why not? The hydrolysis of a phenylalanine-containing peptide is catalyzed by a-chymotrypsin with the following results. Calculate KM and Vmax for the reaction. Peptide Concentration (M) Velocity (M min-1) 2.5 × ...Why can we say that having a pure non- competitive inhibitor present is similar to just having less enzyme present? Distinguish between the concerted and sequential models for the behavior of allosteric enzymes.
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