How does the insertional inactivation of β-galactosidase allow the presence of foreign DNA in a plasmid vector such as pUC19 to be detected?
Answer to relevant QuestionsDescribe two prokaryotic cloning hosts and the beneficial and detrimental features of each. What classes of mammalian proteins are produced by biotechnology? How are the genes for such proteins obtained? How could you detect a colony containing a cloned gene if you did not know the gene sequence but had available purified protein encoded by the gene? You have just discovered a protein in mice that may be an effective cure for cancer, but it is present only in tiny amounts. Describe the steps you would use to produce this protein in therapeutic amounts. Which host would ...What major phenotypic and genotypic properties are used to classify organisms in bacterial taxonomy?
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