If you had a protein X, which is a soluble enzyme found inside the peroxisome, and you wished to separate it from a similar protein Y, which is an enzyme found embedded in the mitochondrial membrane, what would be your initial techniques for isolating those proteins?
Answer to relevant QuestionsWhat is the basis for the separation of proteins by the following techniques? (a) Gel-filtration chromatography (b) Affinity chromatography (c) Ion-exchange chromatography (d) Reverse phase HPLC What is the main difference between reverse phase HPLC and standard ion-exchange or gel filtration chromatography? Would you use a pH meter to monitor the progress of the reaction described in Question 14? Why or why not? Why is it useful to plot rate data for enzymatic reactions as a straight line rather than as a curve? What is a suicide substrate? Why are they important?
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