Show by a series of equations (with structures) the first stage of the Edman method applied to a peptide that has leucine as its N-terminal residue.
Answer to relevant QuestionsWhy can the Edman degradation not be used effectively with very long peptides? With which amino acid sequences would chy-motrypsin be an effective reagent for sequencing the protein from Question 50? Why? What is the point to labeling a secondary an-tibody with a marker that can be visualized instead of just labeling the primary antibody? What are two ways that a compound can be eluted from an affinity column? What could be the advantages or disadvantages of each? An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cation-exchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino ...
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