Somatostatin is a tetradecapeptide of the hypothalamus that inhibits the release of pituitary growth hormone. Its amino

Question:

Somatostatin is a tetradecapeptide of the hypothalamus that inhibits the release of pituitary growth hormone. Its amino acid sequence has been determined by a combination of Edman degradations and enzymic hydrolysis experiments. On the basis of the following data, deduce the primary structure of somatostatin:
1. Edman degradation gave PTH-Ala.
2. Selective hydrolysis gave peptides having the following indicated sequences:
Phe-Trp
Thr-Ser-Cys
Lys-Thr-Phe
Thr-Phe-Thr-Ser-Cys
Asn-Phe-Phe-Trp-Lys
Ala-Gly-Cys-Lys-Asn-Phe
3. Somatostatin has a disulfide bridge.