If you want to purify a DNA-binding protein from a crude mixture of proteins at pH 7,

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If you want to purify a DNA-binding protein from a crude mixture of proteins at pH 7, should you use a DEAE-cellulose or a CM-cellulose column (see Figure 5.A5)? Briefly explain your reasoning.


Figure 5.A5.

H Weak anion exchanger (DEAE) Weak cation exchanger (CM) (X) R Xxn R R Strong anion exchanger ("Q") 6=0

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Biochemistry Concepts And Connections

ISBN: 9780134641621

2nd Edition

Authors: Dean Appling, Spencer Anthony-Cahill, Christopher Mathews

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