Refer to Figure 19.22 (page 632), which describes the base modifications of bacteriophage T4 DNA, and briefly
Question:
Refer to Figure 19.22 (page 632), which describes the base modifications of bacteriophage T4 DNA, and briefly describe some issues that must be dealt with in preparing a restriction map of T4 DNA.
Figure 19.22
Transcribed Image Text:
dCTP H₂O dCTPase 5,10-CH₂-THF THF ATP ADP ATP ADP UDP-glucose ATCGTACTGCAG TAGCATGACGTC PP Cytosine-containing DNA UDP hm-Cytosine 9 dCMP NH₂ dCMP hydroxymethyltransferase CH₂OH hm-dCMP DNases deoxyribose (P) hm-dCDP Deoxyribonucleoside monophosphate kinase hm-dCTP NDP kinase (host cell enzyme) ATHGTAHTGHAG TAGHATGAHGTH hm-Cytosine-containing DNA DNA polymerase 2- and B-glucosyltransferases ATHGTAHTGHAG TAGHATGAHGTH Glucosylated DNA g Glucosyl- hm-cytosin
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Issues in preparing a restriction map of T4 DNA Bacteriophage T4 DNA is heavily modified with about 6 of its cytosine bases hydroxylmethylated and 2 o...View the full answer
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Biochemistry Concepts And Connections
ISBN: 9780134641621
2nd Edition
Authors: Dean Appling, Spencer Anthony-Cahill, Christopher Mathews
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