What would be the pH of the solution described in Question 41?

Question 41

If you mix equal volumes of 0.1 M HCl and 0.20 M TRIS (free amine form; see Table 2.8), is the resulting solution a buffer? Why or why not?

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Table 2.8 Acid and Base Form of Some Useful Biochemical Buffers Acid Form Name N-tris[hydroxymethyl] aminomethane (TRIS) N-tris[hydroxymethyl] methyl-2- aminoethane sulfonate (TES) N 2-hydroxyethylpiperazine-N-2- ethane sulfonate (HEPES) 3 [N-morpholino]propane- sulfonic acid (MOPS) Piperazine-N,N! bis[2-ethanesulfonic acid] (PIPES) TRIS-H+ (protonated form) (HOCH₂), CNH; TES-H+ (zwitterionic form) (HOCH₂) CNH CH₂CH₂SO, HEPES-H* (zwitterionic form) HOCH CHIN NCH.CH.SO, H MOPS-H+ (zwitterionic form) *NCH₂CH₂CH₂SO, H 2-PIPES-H+ (protonated dianion) O,SCH₂CH.N NCH.CH.SO, NCH₂CH.SO, H Base Form TRIS (free amine) (HOCH₂),CNH₂ TES (anionic form) (HOCH₂),CNHCH₂CH₂SO, HEPES (anionic form) HOCH₂CH₂NNCH₂CH.SO, MOPS (anionic form) NCH₂CH₂CH₂SO, 2 PIPES (dianion) O,SCH₂CH.N NCH₂CH.SO, pk₂ 8.3 7.55 7.55 7.2 6.8