Reading from the graphs in the paper by M. M. Santoro and D. W. Bolen, Biochem. 31, 4901-4907 (1992) the following data are obtained for the Gibbs energy change on chemical denaturation (foldedunfolded transition) of E. coli Thioredoxin separately guanidine hydrochloride and urea as a function of the concentrations of.

Further, with both chemical denaturants, the Gibbs energy of unfolding is found to be a linear function of the molarity of the denaturant.

Plot on the same graph the molar extents of the unfolding reaction separately with guanidine hydrochloride and urea as a function of denaturant molarity over the range of 0 to 10 molar.  

Transcribed Image Text:

[M] MGdnHCl = 0 MGdnHCl = 2.5 2.5 Murea = 0 Murea = 6.5 Aunf G (kcal/mol) 7.9 0 9.3 0