The enzyme carbonic anhydrase catalyzes the transformation of carbon dioxide into hydrogen carbonate ions. This reaction was studied by H. DeVoe and G. B. Kistiakowsky (Journal of the American Chemical Society, Vol. 83, p. 274, 1961) and found to obey the Michaelis-Menten model. Use the data below at a given temperature to calculate the maximum rate of the reaction, Ratemax. See Question 45 for the graphical method to use.

Data given in Question 45

According to the Michaelis–Menten model, if 1/ Rate is plotted versus 1/[S], the intercept of the plot (when 1/[S] = 0) is 1/Rate_max. Using the data below at a given temperature, for a given enzyme and its substrate (S), calculate the maximum rate of the reaction, Rate_max.

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[CO₂] (mol/L) 1.3 x 10-3 2.5 x 10-³ 5.0 x 10-3 20.0 × 10-3 Reaction Rate (mol/L . s) 2.8 x 10-5 5.0 x 10-5 8.3 x 10-5 17 x 10-5