Suppose you want to do a physiological experiment that calls for a pH 6.50 buffer. You find that the organism with which you are working is not sensitive to the weak acid H₂X  or its sodium salts.

You have available a 1.0 M solution of this acid and a 1.0 M solution of NaOH. How much of the NaOH solution should be added to 1.0 L of the acid to give a buffer at pH 6.50? Ignore any volume change.

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(Kal = 2 X 10-2 ; K2 = 5.0 × 10-7) 5 Ka2