Draw a schematic diagram that shows a close-up view of two plasma membranes as they come together during cell fusion, as shown in Figure 11–30. Show membrane proteins in both cells that were labeled from the outside by the binding of differently colored fluorescent antibody molecules. Indicate in your drawing the fates of these color tags as the cells fuse. Will the fluorescent labels remain on the outside of the hybrid cell after cell fusion and still be there after the mixing of membrane proteins that occurs during the incubation at 37°C? How would the experimental outcome be different if the incubation were done at 0°C?

Figure 11–30

Transcribed Image Text:

rhodamine- labeled membrane protein fluorescein- labeled membrane protein mouse cell human cell CELL FUSION hybrid cell time = 0 minutes after cell fusion INCUBATION AT 37°C time = 40 minutes after cell fusion