Our electrophoresis sample needs some numbers to make sense of the concentration that can be achieved. Let's assume that we are operating at room temperature, $298 \mathrm{~K}$. We have adjusted the $\mathrm{pH}$ of the solution so that the protein we are trying to separate has a valence charge now of -5 .

a. If the initial protein concentration is $10 \mathrm{mmol}$ and we apply a voltage of +100 volts at the anode and ground $(0 \mathrm{~V})$ the cathode, plot the concentration profile.

b. What is the maximum concentration that can be achieved?