Hamilton, et al., have studied the binding of DNA chromosomes to proteins WT1 and EGR1. WT1 uses a zinc binding site to suppress a certain type of kidney tumor. EGR1 binds to regulate cell proliferation. There may be an important regulatory link between the two proteins.

(a) Use the binding data above to determine the standard state Gibbs energies of reaction at each temperature, the heat of reaction over the temperature range, and the entropy of reaction over the temperature range.

(b) The binding constants are about the same size. Evaluate the relative magnitudes of the enthalpy and entropy of formation for each binding type. Does the thermodynamic analysis imply that the binding types are similar or different?

Transcribed Image Text:

Ink at 4°C Ink at 11°C Ink at 37°C WT1 19.6 20 20.9 EGR1 20.4 20 19