T. Svedberg measured the molecular weight of carbonyl hemoglobin (specific volume = 0.755 mL g 1 ,

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T. Svedberg measured the molecular weight of carbonyl hemoglobin (specific volume = 0.755 mL g−1, D = 7.00 × 10–11 m2 s–1) by velocity centrifugation. In this experiment, 0.960 g of protein were dissolved in 100. mLof water at 303 K (ρ = 0.998 g cm3), and the solution was spun at 39,300 rpm. After 30.0 min, the concentration boundary advanced by 0.0740 cm from an initial position of 4.525 cm. Calculate the molecular weight of carbonyl hemoglobin.

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Physical Chemistry

ISBN: 978-0321812001

3rd edition

Authors: Thomas Engel, Philip Reid

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