As part of the Human Genome Mapping Project, you are trying to clone a gene involved in colon cancer. Your first step is to localize the gene using RFLP markers. In the following table, RFLP loci are defined by STS number (e.g., STS1), and the gene for colon cancer is designated C.

(a) Given the percentage recombination between different RFLP loci and the gene for colon cancer shown in the table, draw a genetic map showing the order and genetic distances between adjacent RFLP markers and the gene for colon cancer. 

(b) Given that the human genome contains approximately 3.3 × 10⁹ base pairs of DNA and that the human genetic map contains approximately 3300 cM, approximately how many base pairs of DNA are located along the stretch of chromosome defined by this RFLP map? 

(c) How many base pairs of DNA are present in the region between the colon cancer gene and the nearest STS?

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% Recombi- % Recombi- nation Loci Loci nation C, STS1 C, STS2 C, STS3 STS1, STS5 50 10 STS2, STS3 15 30 15 STS2, STS4 14 C, STS4 C, STS5 STS1, STS2 STS2, STS5 50 1 STS3, STS4 16 40 STS3, STS5 50 25 STS1, STS3 STS4, STS5 35 41 STS1, STS4 50