Two additional mutations in the trp A gene of E. coli, trp A58 and trp A487, were

Question:

Two additional mutations in the trp A gene of E. coli, trp A58 and trp A487, were ordered relative to trp A223 and the outside marker anth by three-factor transduction crosses as described in Problem 8.18. The results of these crosses are summarized in the following table. What is the linear order of anth and the three mutant sites in the trp A gene?

anth Allele in trp+ Recombinants Donor Recipient Markers % Cross Markers anth- 82 anth-- anth+- 72 anth*: A487 A223 332