a. UHPLC can provide exquisite resolution when run on long columns at high pressure or rapid separations

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a. UHPLC can provide exquisite resolution when run on long columns at high pressure or rapid separations with reasonable resolution if short columns are run fast. The drug acetaminophen run on a 50x2.1 mmC18 50x2.1 mmC18 is UHPLC column has a retention time of 0.63 min 0.63 min and a full width at half-height of 2.3 s. 2.3 s. Find the plate number and plate height. How many 1.7-μm-diameter 1.7-μm-diameter particles placed side-by-side are equal to one theoretical plate?

b. From Figure 25-3, we expect an optimum plate height of 4μm. 4μm. hi How many particles placed side-by-side are equal to one theoretical plate? Do you think the column in (a) is being run for maximum resolution or maximum speed?

Figure 25-3

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Quantitative Chemical Analysis

ISBN: 9781319164300

10th Edition

Authors: Daniel C. Harris, Charles A. Lucy

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