Forster resonance energy transfer. Spectra of six dyes are shown here. Select a suitable donor/acceptor pair for

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Forster resonance energy transfer. Spectra of six dyes are shown here. Select a suitable donor/acceptor pair for each analytical objective.

a. Excitation at 488 nm 488 nm results in little fluorescence at 600 nm 600 nm when the dyes are apart and strong fluorescence at 600 nm 600 nm when they are close.
b. Excitation at 488 nm 488 nm results in no fluorescence when the dyes are close and strong fluorescence at 520 nm 520 nm when they are apart.
c. Excitation at 532 nm 532 nm results in strong fluorescence at 670 nm 670 nm when the dyes are close, and the fluorescence shifts to 570 nm 570 nm when the dyes are apart.

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Quantitative Chemical Analysis

ISBN: 9781319164300

10th Edition

Authors: Daniel C. Harris, Charles A. Lucy

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