Tandem mass tags. Tags A, B, and C in Figure 22-41 with nominal m/z 116 m/z 116

Question:

Tandem mass tags. Tags A, B, and C in Figure 22-41 with nominal m/z 116 m/z 116 reporter ions are members of a set of 1212 tags accessible by certain synthetic pathways.

a. Explain the functions of the amine-reactive group, the balance COCO group, and the reporter in the structure at the top left of Figure 22-41.

b. Ions selected by Q1 in a tandem mass spectrometer all have nominal m/z 146. m/z 146. Which chemical bond of a labeled peptide breaks to give m/z 146?m/z 146? Why is it necessary for all mass tags to produce a fragment with the same nominal mass (m/z 146) (m/z 146) at the Q1 stage of analysis?

c. The published set of mass tags with the general structure in Figure 22-41 has two reporters with nominal m/z 115, m/z 115, three with nominal m/z 116,m/z 116, three with nominal m/z 117,m/z 117, and four with nominal m/z 118. m/z 118. Draw one isotopologue of the m/z 146 m/z 146 ion C8H16NO+ C8H16NO+ that produces a reporter with m/z 115,m/z 115, one that produces m/z 117,m/z 117, and one that produces m/z 118.m/z 118. The top left structure in Figure 22-41 shows which sites could be labeled with 2H,2H 13C,13C, 15N,15N, or 180.18O.

Figure 22-41

Fantastic news! We've Found the answer you've been seeking!

Step by Step Answer:

Related Book For  book-img-for-question

Quantitative Chemical Analysis

ISBN: 9781319164300

10th Edition

Authors: Daniel C. Harris, Charles A. Lucy

Question Posted: