1. Bonnie the Biochemist is trying to purify two proteins from a bacterial cell culture. After several...
Question:
1. Bonnie the Biochemist is trying to purify two proteins from a bacterial cell culture. After several steps, she has succeeded in isolating a mixture of two proteins: protein X and protein Y. a bacterial photo receptor protein. Protein Y is contently linked to an organic ring structure that strongly absorbs light in the orange region of the spectrum (thus. the protein is green). The pit at which each protein has a net charge of protein X and 6.7 for protein Y. Both of zero is pH 7.8 for these proteins are unusually stable over a wide range of pHs, from pi 12 to pH 12. If Ronnie presented you with the following data from gel filtration and SDS-PAGE, what conclusions could you reach concerning the molecular weight and sub unit composition of each protein? (I'm sure she'd want justification for your hypothesis!)
2. An enzyme has the following kinetic characteristics: K m = 200 mM and k cat = 1.9* 10 -2 sec -1 . At V max , this enzyme would be how much faster that an enzyme with an S ? A mutation at positon 107 that has the following kinetic characteristics: K M = 400 mM and k cat = 5*10 -4 sece -1 ?
Concepts in Federal Taxation 2017
ISBN: 9781305965119
24th edition
Authors: Kevin E. Murphy, Mark Higgins