Read the case study “MOLECULAR PATHOGENESIS OF MICROBIAL INFECTION” and write a paper about it.
1. The authors use ChIP, or chromatin immunoprecipitation, combined with RT-qPCR., describe the principle of chromatin precipitation and discuss its usage in this study in your own words
2. Explain the purpose of the strains that harbor pPL2-Phly-lux and pPL2-Phly-lux-codY shown in Fig. 1.C
3. Referring to the appropriate figure, explain how CodY interacts with prfA and ilvD regions in the presence or absence of BCAA.
4. In Your Own Words, And Referring To Figures In The Paper Where Appropriate, Describe How CodY Contributes To Regulation Of Virulence Gene Expression In Listeria Monocytogenes
5. Imagine you are a researcher who is working in a laboratory that has recently identified and sequenced the genome of a new pathogen. Based on the characteristics of the microorganism, it is similar to L. monocytogenes and may contain many of the same genes. Describe what approach you would take to determine if the new pathogen contained genes that could encode codY and prfA

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Molecular Pathogenesis of Microbial Infection 1 The authors use ChIP or chromatin immunoprecipitation combined with RTqPCR describe the principle of chromatin precipitation and discuss its usage in th... View the full answer