you wish to capture 3 micro m particles in a linear density gradient having a density gradient having a density of 1.12 g/ cm^3 at the bottom and 1.00 at the top .You layer a thin particle suspension on the top of the 6 cm column of fluid with a viscosity of 1.0 cp and allow particles to settle at 1g

a.How long must wait for the particles you want (density=1.07 g/cm^3) to sediment to within 0.1 cm of their isopycnic level?Is it possible to determine the time required for the particles to sediment to exactly their isopycnic level?
b.If instead of 1g you use a centrifuge running at 800 rpm,and the top of the fluid is 5 cm from the center of rotation ,how long must it centrifuge for the particles to move to within .1 cm of their isopycnic level?

Answer rating: 100% (QA)

aIt would take approximately 9 minutes for the particles to sediment to within 01 cm of their isopycnic level It is not possible to determine the time ... View the full answer