1. The column chromatography procedure for the separation of a polar and a nonpolar compound calls...

 

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1. The column chromatography procedure for the separation of a polar and a nonpolar compound calls for sequential elution with the following solvents: hexane, 70:30 hexane : acetone, acetone and 80:20 acetone methanol. Why it is important to follow this solvent order? 2. During a mixture separation with column chromatography, why must the level of the solvent be kept above the top of the stationary phase once the procedure is started? 3. Why did ß-carotene move through the column faster than other components (mostly green and gray), regardless which solvent is used to elute them? [Use structural ideas in your answer and consider the column absorbent.] 1. The column chromatography procedure for the separation of a polar and a nonpolar compound calls for sequential elution with the following solvents: hexane, 70:30 hexane : acetone, acetone and 80:20 acetone methanol. Why it is important to follow this solvent order? 2. During a mixture separation with column chromatography, why must the level of the solvent be kept above the top of the stationary phase once the procedure is started? 3. Why did ß-carotene move through the column faster than other components (mostly green and gray), regardless which solvent is used to elute them? [Use structural ideas in your answer and consider the column absorbent.]

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