Assuming the product formed from your enzyme has an absorbance at 405 nm, the following data...

  

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Assuming the product formed from your enzyme has an absorbance at 405 nm, the following data for constructing a standard curve were obtained. *The concentration of Product Stock solution: 50 mM. Tube [Product] Product Stočk Buffer Absorbance (mM) solution* (mL) (405 nm) (mL) 0.21 10:32 O a 4) E ENG 15/06/2021 The concentration of Product Stock solution: 50 mM. Tube [Product] Product Stock Buffer Absorbance (mM) solution* (mL) (405 nm) (mL) 0. 2 4 0.21 3 1.5 3.5 0.35 4 3 0.48 3.5 1.5 0.83 1.12 9. mpletion Status: 20 3D 40 50 60 7D 9. 10 11 12 130 150 16 170 18 19 20 22 23 21 24 25 3.5 1.5 0.83 6. 1.12 (i) Calculate the concentration of product [Product] in each tube used to generate the standard curve. Type your answers directly into the answer window. Note: you do NOT need to reproduce the table, just indicate the tube number and concentration. [2 marks] (ii) Produce a standard curve using this dataset (you may use excel). Generate a trendline (set y-intercept to zero) and type the equation of the line into the answer window. Note: you do NOT need to show the standard curve you produced, only the equation of the trendline is required. [2 marks] (iii) Use the equation you generated from part (ii) to estimate the concentration of product in an unknown sample, given that the Abs at 405 nm of this unknown sample was found to be 0.67. Type your answer into the answer window. [1 marks] Note: Type your answers into the answer window. Please clearly show which answer is for each part by including (i), (i1), or (iii) before your answer For the toolbar, press ALT-F10 (PC) or ALT+FN+F10 (Mac). ... Arial 14px B IUS Paragraph 10:32 4) E ENG 15/06/2021 earch 19 18 !!! 14 5. Assuming the product formed from your enzyme has an absorbance at 405 nm, the following data for constructing a standard curve were obtained. *The concentration of Product Stock solution: 50 mM. Tube [Product] Product Stočk Buffer Absorbance (mM) solution* (mL) (405 nm) (mL) 0.21 10:32 O a 4) E ENG 15/06/2021 The concentration of Product Stock solution: 50 mM. Tube [Product] Product Stock Buffer Absorbance (mM) solution* (mL) (405 nm) (mL) 0. 2 4 0.21 3 1.5 3.5 0.35 4 3 0.48 3.5 1.5 0.83 1.12 9. mpletion Status: 20 3D 40 50 60 7D 9. 10 11 12 130 150 16 170 18 19 20 22 23 21 24 25 3.5 1.5 0.83 6. 1.12 (i) Calculate the concentration of product [Product] in each tube used to generate the standard curve. Type your answers directly into the answer window. Note: you do NOT need to reproduce the table, just indicate the tube number and concentration. [2 marks] (ii) Produce a standard curve using this dataset (you may use excel). Generate a trendline (set y-intercept to zero) and type the equation of the line into the answer window. Note: you do NOT need to show the standard curve you produced, only the equation of the trendline is required. [2 marks] (iii) Use the equation you generated from part (ii) to estimate the concentration of product in an unknown sample, given that the Abs at 405 nm of this unknown sample was found to be 0.67. Type your answer into the answer window. [1 marks] Note: Type your answers into the answer window. Please clearly show which answer is for each part by including (i), (i1), or (iii) before your answer For the toolbar, press ALT-F10 (PC) or ALT+FN+F10 (Mac). ... Arial 14px B IUS Paragraph 10:32 4) E ENG 15/06/2021 earch 19 18 !!! 14 5.

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