A biochemist replaces the DNA-binding domain of the yeast Gal4 protein with the DNA-binding domain from the
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Question:
A biochemist replaces the DNA-binding domain of the yeast Gal4 protein with the DNA-binding domain from the Lac repressor and finds that the engineered protein no longer regulates transcription of the GAL genes in yeast. Draw a diagram of the different functional domains you would expect to find in the Gal4 protein and in the engineered protein. Why does the engineered
protein no longer regulate transcription of the GAL genes? What might be done to the DNA-binding site recognized by this chimeric protein to make it functional in activating transcription of GAL genes?
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