An HPLC method was develop for the separation and determination of ibuprofen in rat plasma samples as
Question:
An HPLC method was develop for the separation and determination of ibuprofen in rat plasma samples as part of a study of the time course of the drug in laboratory animals. Several standards were chromatographed and the following results obtained:
Next, a 10 mg/kg sample of ibuprofen was administered orally to a laboratory rat. Blood samples were drawn at various times after administration of the drug and subjected to HPLC analysis. The following results were obtained:
Find the concentration of ibuprofen in the blood plasma for each of the times given above and plot the concentration versus time. On a percentage basis, during what half-hour period (1 st , 2 nd , 3 rd etc) is most of the ibuprofen lost?
An Introduction to Statistical Methods and Data Analysis
ISBN: 978-1305269477
7th edition
Authors: R. Lyman Ott, Micheal T. Longnecker