Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4.7HO, 0.5; KHPO4, 1; KC1, 0.5 and...
Fantastic news! We've Found the answer you've been seeking!
Question:
Transcribed Image Text:
Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4.7H₂O, 0.5; K₂HPO4, 1; KC1, 0.5 and supplemented with 1 mL FeSO5.7H2O as trace element. The medium was adjusted to pH 5.5 and inoculated aseptically by adding Aspergillus terreus spore suspension (approximately 2x107 spores/mL) to 50 mL of sterilized medium containing 5% (w/v) sugarcane bagasse in a 250 mL Erlenmeyer flask and incubated at 35°C for 24 h at 200 rpm. Table 1 shows the results obtained for media optimization before proceeding to statistical optimization. Table 1: Effect of different bagasse and yeast extract concentrations on cellulase enzyme activity of Aspergillus terreus Concentration Bagasse (%) 1 2 3 4 5 6 Yeast extract (g L-¹) 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 Endoglucanase activity (U mL-¹) 0.32 0.47 0.65 0.88 0.96 0.73 1.40 1.90 2.47 1.99 1.67 1.51 1.35 1.18 074 Protein content (mg mL-¹) 0.90 1.83 2.50 3.16 3.30 1.88 2.17 1.82 2.04 2.70 2.68 3.10 3.76 3.20 2.52 Specific activity (U mg-¹ protein) 0.35 0.25 0.26 0.28 0.29 0.39 0.65 1.04 1.21 0.74 0.63 0.48 0.35 0.37 0.29 Explain the medium used is either synthetic or complex medium for fermentation production. Medium used for cellulase production contained (g/L): NaNO3, 3; MgSO4.7H₂O, 0.5; K₂HPO4, 1; KC1, 0.5 and supplemented with 1 mL FeSO5.7H2O as trace element. The medium was adjusted to pH 5.5 and inoculated aseptically by adding Aspergillus terreus spore suspension (approximately 2x107 spores/mL) to 50 mL of sterilized medium containing 5% (w/v) sugarcane bagasse in a 250 mL Erlenmeyer flask and incubated at 35°C for 24 h at 200 rpm. Table 1 shows the results obtained for media optimization before proceeding to statistical optimization. Table 1: Effect of different bagasse and yeast extract concentrations on cellulase enzyme activity of Aspergillus terreus Concentration Bagasse (%) 1 2 3 4 5 6 Yeast extract (g L-¹) 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 Endoglucanase activity (U mL-¹) 0.32 0.47 0.65 0.88 0.96 0.73 1.40 1.90 2.47 1.99 1.67 1.51 1.35 1.18 074 Protein content (mg mL-¹) 0.90 1.83 2.50 3.16 3.30 1.88 2.17 1.82 2.04 2.70 2.68 3.10 3.76 3.20 2.52 Specific activity (U mg-¹ protein) 0.35 0.25 0.26 0.28 0.29 0.39 0.65 1.04 1.21 0.74 0.63 0.48 0.35 0.37 0.29 Explain the medium used is either synthetic or complex medium for fermentation production.
Expert Answer:
Answer rating: 100% (QA)
Solution Carbom source used for cellulose production are Vrius sures f rbn suh s strh frutse mltse n... View the full answer
Related Book For
Posted Date:
Students also viewed these chemistry questions
-
In problem 8.16, a college chemistry instructor thinks the use of embedded tutors will improve the success rate in introductory chemistry courses. The instructor carried out a hypothesis test and...
-
You have exams in economics and chemistry coming up, and you have 5 hours available for studying. The following table shows the trade-offs you face in allocating the time you will spend studying each...
-
A college chemistry instructor thinks the use of embedded tutors will improve the success rate in introductory chemistry courses. The passing rate for introductory chemistry is 62%. During one...
-
(a) Find the acceleration at time t = 5 min of a helicopter whose height is s(t) = 300t 4t 3 m. (b) Plot the acceleration s" for 0 t 6. How does this graph show that the helicopter is slowing down...
-
How is the changing age structure of the population likely to affect consumer loan programs? What other forces are reshaping household lending today?
-
Business communicators are encouraged to profile or visualize the audience for their messages. How is this possible if you dont really know the people who will receive a sales letter or who will hear...
-
Rhys Hoskins gets a walk in his next plate appearance. Use the following table of the top 15 players by number of plate appearances (PA) in the 2019 Major League Baseball season to assign empirical...
-
Considering the stress concentration at point A in the figure, determine the maximum normal and shear stresses at A if F = 200 lbf. .y 2 in 0 12 in I-in dia. g-in R. 2 in 1-in dia. 15 in 1-in dia.
-
Search Nordstrom Inc's 10-K for segment disclosures. List the segments for which the company reports results of operations. What characteristics does the firm use to differentiate segments? Does this...
-
A manager of a store that sells and installs spas wants to prepare a forecast for January, February, and March of next year. Her forecasts are a combination of trend and seasonality. She uses the...
-
Test and debug the program Specifications Create a list of valid entries and the correct results for each set of entries. Then, make sure that the results are correct when you test with these...
-
Agent Johnny Utah the former Motocross and Extreme athlete is now with the FBI Bank Robbery Task Force and investigating Bank robberies committed by the Ex-Presidents. Utah and his partner , Angelo...
-
In 1857, the Green Ridge Tribal Nation signed a treaty reserving for itself a portion of land from its aboriginal landholdings. The treaty was signed by the Nation as well as the United States and...
-
Find a linear equation for the line that passes through (7,5) and (3,17)
-
Daniel White recorded the following transactions during the month of April. Apr. 3 Cash 2,480 Service Revenue 2,480 16 Rent Expense 490 Cash 490 20 20 Salaries and Wages Expense 180 Cash 180 Post...
-
You shake a slinky continuously in an up and down fashion such that a transverse waves propagate horizontally down its length. If you count 5 peaks over a horizontal distance of 2 . 5 1 m , what is...
-
Mira Mesa Appliances makes and sells kitchen equipment for offices and hotel rooms. Mira Mesa management believes that a new model of refrigerator made out of a synthetic material would sell well at...
-
Show that the block upper triangular matrix A in Example 5 is invertible if and only if both A 11 and A 22 are invertible. Data from in Example 5 EXAMPLE 5 A matrix of the form A = [ A11 A12 0 A22 is...
-
Dehydrohalogenation of the diastereomeric forms of 1-chloro-1,2-diphenylpropane is stereospecific. One diastereomer yields (E )-1,2-diphenylpropene, and the other yields the Z isomer. Which...
-
Multifidene is a sperm cell-attracting substance released by the female of a species of brown algae (Cutleria multifida). The constitution of multifidene is (a) How many stereoisomers are represented...
-
In the presence of the enzyme aconitase, the double bond of aconitic acid undergoes hydration. The reaction is reversible, and the following equilibrium is established: (a) The major tricarboxylic...
-
Discuss the effect on setting performance bonuses for staff if financial performance for a period contains both realised and unrealised gains/losses.
-
Financial statements should reflect realistically the performance and position of an organisation, but most of the accountant's rules conflict directly with the concept of realism. Discuss.
-
Explain why financial reports prepared under the historical cost convention are subject to the following major limitations: inventory is undervalued the depreciation charge to the income statement...
Study smarter with the SolutionInn App