1. Describe restriction endonucleases? Give examples of commonly used restriction endonucleases.
2. What is restriction site?
3. Define transformation.
4. Define plasmids. Give examples
5. Describe molecula cloning. Step by step. You can illustrate with diagrams.
6. Give 5 examples of sources of genetic material such as blood for testing. Make comments.
7. What are the differences between NGS and Sanger sequencing.
8. Describe methods for scanning a gene for mutations before sequencing. Write down advantages and disadvantages.
9. Unclassified variants are a problem for genetic testing. Please list the suggestions fort his problem to be solved.
10. Summarise 3 methods for testing for a specific mutation or SNP.
11. Describe recombinant DNA technology?
12. What are the main goals of recombinant DNA technology?
13. List the tools in recombinant DNA technology.
14. Describe CLONING methodology.
15. What are cloning vectors. Why plasmids are good cloning vectors.
16. What are the disadvantages using plasmids?
17. What are the applications of recombinant DNA technology?
18. Define ethics and safety in recombinant DNA technology. Describe ethical issues.
19. Describe PCR DNA Sequencing.
20. Describe FISH.
21. Describe Microarray technology
22. What is genome editing?

 

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Question 1 Restriction endonucleases REs are bacterial enzymes that cleave doublestranded DNA Type I REs are important in bacterial function but do not cleave DNA at specific sequences Type II REs des... View the full answer