For an electrophoretic mobility shift assay, you mix in a test tube bacterial cell extract and a
Fantastic news! We've Found the answer you've been seeking!
Question:
For an electrophoretic mobility shift assay, you mix in a test tube bacterial cell extract and a 32P- labeled fragment of DNA that contains the CAP binding site and the lacPof the lac operon. It does not contain lacO or any structural genes. What would cause a mobility shift if added to the mix and what would cause a second mobility shift if added to the same mix next.
I know cAMP is responsible for the first mobility shift, but of these choices, I am unsure of what causes the second mobility shift (only one of these choices is correct)
a) glucose
b) lactose
c) allolactose
d) cAMP
e) anti-lac repressor antibody
f) anti-RNA polymerase antibody
Related Book For
Posted Date: