I just need question number three. Calculating Transformation Efficiency por miorogram of prote TE = (# of

I just need question number three.

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Calculating Transformation Efficiency por miorogram of prote TE = (# of transformed colonies)/(ug of DNA) Use the results in the table below to calculate the TE for this experiment. For your calculations, assume that 10 uL of plasmid was used in the experiment and the plasmid concentration was 0.2 ug/uL. Cells Plate LB LB +Amp LB +Amp pGLO+ LB +Amp +Arabinose pGLO- # of Colonies TNTC 0 256 124 # of Glowing Colonies 0 0 0 124 Your discussion post must include: 1. Calculated TE 2. Identify which results you used in your calculation and why 3. Describe the purpose for each of the plates used. For example, what was the purpose of platting PGLO- cells on the LB plate? Calculating Transformation Efficiency por miorogram of prote TE = (# of transformed colonies)/(ug of DNA) Use the results in the table below to calculate the TE for this experiment. For your calculations, assume that 10 uL of plasmid was used in the experiment and the plasmid concentration was 0.2 ug/uL. Cells Plate LB LB +Amp LB +Amp pGLO+ LB +Amp +Arabinose pGLO- # of Colonies TNTC 0 256 124 # of Glowing Colonies 0 0 0 124 Your discussion post must include: 1. Calculated TE 2. Identify which results you used in your calculation and why 3. Describe the purpose for each of the plates used. For example, what was the purpose of platting PGLO- cells on the LB plate?

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In this study pGLO plasmid contains Ampicillin resistance gene and other genes to be cloned Cells ha... View the full answer