Internal standards are commonly used in chromatography to calculate the concentration of an analyte. For any...
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Internal standards are commonly used in chromatography to calculate the concentration of an analyte. For any particular detector, the relative response factor for the analyte compared to the internal standard must be determined first. Calibrating the linearity of the response factor for an analyte compared to the internal standard requires one to make a series of solutions with the same concentration of standard, and a varying concentration of analyte. A plot of the response of the analyte relative to the standard (peak area of analyte/peak area of standard) versus the concentration of the analyte relative to the standard ([analyte]/[standard]) should produce a linear response with a slope equal to the response factor, F. area of analyte signal concentration of analyte area of standard signal concentration of standard The table contains data for a chromatographic analysis of an analyte using an internal standard. Peak areas of each are measured by a mass spectrometer. The volume injected for Standard Analyte Standard Analyte concentration concentration peak (M) pea Sample (M) area area each sample is similar, but not exactly the same. 1.00 10.0 309 2991 Calculate the peak area ratio (analyte/standard) and the concentration ratio [analyte]/[standard] for each of the samples. Then, calculate F for each sample (peak area ratio/concentration ratio). 5.00 10.0 3677 6203 3 10.0 10.0 3025 2811 Internal standards are commonly used in chromatography to calculate the concentration of an analyte. For any particular detector, the relative response factor for the analyte compared to the internal standard must be determined first. Calibrating the linearity of the response factor for an analyte compared to the internal standard requires one to make a series of solutions with the same concentration of standard, and a varying concentration of analyte. A plot of the response of the analyte relative to the standard (peak area of analyte/peak area of standard) versus the concentration of the analyte relative to the standard ([analyte]/[standard]) should produce a linear response with a slope equal to the response factor, F. area of analyte signal concentration of analyte area of standard signal concentration of standard The table contains data for a chromatographic analysis of an analyte using an internal standard. Peak areas of each are measured by a mass spectrometer. The volume injected for Standard Analyte Standard Analyte concentration concentration peak (M) pea Sample (M) area area each sample is similar, but not exactly the same. 1.00 10.0 309 2991 Calculate the peak area ratio (analyte/standard) and the concentration ratio [analyte]/[standard] for each of the samples. Then, calculate F for each sample (peak area ratio/concentration ratio). 5.00 10.0 3677 6203 3 10.0 10.0 3025 2811
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Related Book For
Chemistry The Central Science
ISBN: 978-0321696724
12th edition
Authors: Theodore Brown, Eugene LeMay, Bruce Bursten, Catherine Murphy, Patrick Woodward
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