The most reproducible and accurate method of determining the amount of filamentous actin (F-actin) content versus free globular-actin (G-actin) content in a cell population is to use Western blot quantitation of F-actin and G-actin cellular fractions.

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Total protein normalization is a useful method for obtaining accurate quantitative western blotting data as housekeeping proteins can often be affected by experimental conditions The Invitrogen NoStai... View the full answer