Number 10 NAD+ NADH Extinction Coefficient 260 nm Carotene Vitamin A 18,000 15,000 Why are data at

Number 10

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NAD+ NADH Extinction Coefficient 260 nm Carotene Vitamin A 18,000 15,000 Why are data at two wavelengths needed to solve this problem? 10. Ten grams of butter were saponified and the nonsaponifiable fraction extracted into 25 ml of chloroform. The optical density of the chloroform solution in a 1 cm cuvette was 0.53 at 328 nm and 0.48 at 458 nm. Calculate the carotene and vitamin A content of the butter. The extinction coefficients of carotene and vitamin A at the two waelengths are given below: 21% in CHCI 328 nm 340 1,550 340 nm 0 6,220 458 nm 2,200 0 (Be careful of units!) 11. A solution containing two substances, A and B, has an absorbance in a 1 cm cuvette of 0.36 at 350 nm and 0.225 at 400 nm. The molar extinction coefficients of A and B at the two wavelengths are given below. Calculate the concentrations of A and B in the solution. tion NAD+ NADH Extinction Coefficient 260 nm Carotene Vitamin A 18,000 15,000 Why are data at two wavelengths needed to solve this problem? 10. Ten grams of butter were saponified and the nonsaponifiable fraction extracted into 25 ml of chloroform. The optical density of the chloroform solution in a 1 cm cuvette was 0.53 at 328 nm and 0.48 at 458 nm. Calculate the carotene and vitamin A content of the butter. The extinction coefficients of carotene and vitamin A at the two waelengths are given below: 21% in CHCI 328 nm 340 1,550 340 nm 0 6,220 458 nm 2,200 0 (Be careful of units!) 11. A solution containing two substances, A and B, has an absorbance in a 1 cm cuvette of 0.36 at 350 nm and 0.225 at 400 nm. The molar extinction coefficients of A and B at the two wavelengths are given below. Calculate the concentrations of A and B in the solution. tion

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Solution contains carotene and vitamin A Length of cuvette l 1 cm In 2 chloroform At 328 nm Molar ex... View the full answer