O Attempt 2 Question 7 of 18 To assist in completing this question, you may reference...
Fantastic news! We've Found the answer you've been seeking!
Question:
Transcribed Image Text:
O Attempt 2 Question 7 of 18 To assist in completing this question, you may reference the Animated Technique Video - Gel Electrophoresis (SDS PAGE). A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first lane in each gel is a molecular weight ladder. Native PAGE Non-reducing SDS-PAGE Reducing SDS-PAGE 1000 kDa 250 kDa 250 kDa 500 kDa 150 kDa 150 kDa 250 kDa 100 kDa 100 kDa 100 kDa 60 kDa 60 kDa 50 kDa 50 kDa 50 kDa 30 kDa 30 kDa 30 kDa Answer Bank 250 kDa 100 kDa 100 kDa 100 kDa 60 kDa 60 kDa 50 kDa 50 kDa 50 kDa 30 kDa 30 kDa 30 kDa Answer Bank What are the molecular weights of the protein X polypeptides? 60 kDa and 30 kDa Incorrect What are the molecular weights of the protein Y polypeptides? 60 kDa, 50 kDa, and 30 kDa Incorrect O Attempt 2 Question 7 of 18 To assist in completing this question, you may reference the Animated Technique Video - Gel Electrophoresis (SDS PAGE). A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first lane in each gel is a molecular weight ladder. Native PAGE Non-reducing SDS-PAGE Reducing SDS-PAGE 1000 kDa 250 kDa 250 kDa 500 kDa 150 kDa 150 kDa 250 kDa 100 kDa 100 kDa 100 kDa 60 kDa 60 kDa 50 kDa 50 kDa 50 kDa 30 kDa 30 kDa 30 kDa Answer Bank 250 kDa 100 kDa 100 kDa 100 kDa 60 kDa 60 kDa 50 kDa 50 kDa 50 kDa 30 kDa 30 kDa 30 kDa Answer Bank What are the molecular weights of the protein X polypeptides? 60 kDa and 30 kDa Incorrect What are the molecular weights of the protein Y polypeptides? 60 kDa, 50 kDa, and 30 kDa Incorrect
Expert Answer:
Answer rating: 100% (QA)
this Answer SDB NonReduing Native PAGE PAGE Reducing SosPAGE 1000 KDa 250KDA 250KDE 500 KD 2... View the full answer
Related Book For
Brock Biology of Microorganisms
ISBN: 978-0321897398
14th edition
Authors: Michael Madigan, John Martinko, Kelly Bender, Daniel Buckley
Posted Date:
Students also viewed these chemistry questions
-
A protein gives a single band on SDS gel electrophoresis, as shown in lanes 1 and 2 below. There is little if any effect from adding β -mercaptoethanol (BME) to the sample; if anything,...
-
A 25.0-mL sample of sodium sulfate solution was analyzed by adding an excess of barium chloride solution to produce barium sulfate crystals, which were filtered from the solution. Na2SO4(aq) +...
-
A mixture contained aluminum sulfate and sodium sulfate. A sample of the mixture weighing 3.458 g was dissolved in water and treated with sodium hydroxide solution to yield a precipitate. The...
-
What is the present value of $9,000 received: a. Twenty eight years from today when the interest rate is 10% per year? b. Fourteen years from today when the interest rate is 10% per year? c. Seven...
-
A tie-down on the deck of a sailboat consists of a bent bar bolted at both ends, as shown in the figure. The diameter dB of the bar is ¼ in, the diameter dW of the washers is 7/8 in, and the...
-
Trek has designed a new off-road bicycle designed to stand up to the rugged conditions of trail riding. Develop a theme for an advertising strategy that ensures all three components of attitude are...
-
Derive equation (4.1) for the total sample of the two-sample t-test. Data from Equation 4.1 n = 2 40 (21-a/2 + 21-3)
-
The cost of goods sold computations for Target Company and Caesar Company are shown below. Instructions(a) Compute inventory turnover and days in inventory for each company.(b) Which company moves...
-
A project has an initial cost of $35,000, expected net cash inflows of $14,000 per year for 11 years, and a cost of capital of 10%. What is the project's PI? (Hint: Begin by constructing a time...
-
Skillings Industrial Chemicals, Inc., operates a refinery in southwestern Ohio near the Ohio River. The companys primary product is manufactured from a chemical process that requires the use of two...
-
let x, X, X be a random sample of size n from a normal distribution with mean u and variance o then what is the mean of y
-
Explain why in recent years European banks turned their focus back to their national markets. Outline the potential risks associated with this process. Q2: Explain how the securitization technique...
-
loan to the value of R1 000 000 is repaid over a period of 5 years at an interest rate of 12%p.a. compounded monthly. How much capital is still outstanding after 3 years (36 payments)?
-
Calculate his monthly (P+I) payment assuming the mortgage factor is $5.68 per $1,000
-
Sales in 2020 $50M, 2021 $55M, 2022 $60M; COGS $30M, $32M, $34M respectively; SG&A $10M, $13M, $16M; Operating Expenses $7M, $8M, $9M Calculate Operating Margin and Operating Profit for each year....
-
Krieger Krisp Donuts, Inc. acquired 40% of the voting common shares of Delevan Bakers Corporation on January 1, 2022, at a total cost of $2,535,000. At acquisition, the reported book value of Delevan...
-
31) For each type of report listed below, identify one planning decision and one controlling decision for which the information would be helpful. Assume you are a Walgreen Company store. a.annual...
-
Find a polar equation for the curve represented by the given Cartesian equation. 4y 2 = x
-
Why is investigation of the metabolome lagging behind that of the proteome?
-
Contrast the enzyme(s) present in the virions of a retrovirus and a positive-strand RNA bacteriophage. Why do they differ if each has plus configuration single-stranded RNA as its genome?
-
What are the major differences between oxygenic and anoxygenic phototrophs?
-
Sketch and explain a simple diagram to match your exhibit.
-
a. Write out and explain the equation for volume breakeven. b. What is the difference between accounting breakeven and economic breakeven?
-
a. What is profit analysis, also known as cost-volume-profit (CVP) analysis? b. Why is profit analysis so useful to healthcare managers? c. What is a profit and loss (P&L) statement?
Study smarter with the SolutionInn App